Glyphosate (GLY), the most widely used herbicide worldwide, has been linked to neurological dysfunctions, while Pituitary adenylate cyclase-activating polypeptide (PACAP) exhibits neuroprotective properties. This short report investigates the effects of GLY exposure and PACAP treatment on neurobehavioral functions in Swiss albino mice. Behavioral assessments including Tail Suspension Test, Dark/Light Box, Elevated-Plus-Maze, and Open Field Test revealed that repeated GLY exposure induces anxiety- and depression-like behaviors, whereas co-treatment with PACAP alleviates these effects. Our findings highlight PACAP as a potential therapeutic candidate against glyphosate-induced neurobehavioral impairments.